发展分子转子型荧光染料，开发核酸、蛋白质等生物大分子的荧光标记技术，实现活细胞及活体动物的特异性荧光成像，为生物大分子原位可视化研究提供新颖的荧光探针工具，同时也为生物传感与疾病诊断提供新技术。

代表性论文

(1) Li Jiang#, Xin Xie#, Ni Su#, Dasheng Zhang#, Xianjun Chen#, Xiaochen Xu#, Bibi Zhang, Kaiyi Huang, Jingwei Yu, Mengyue Fang, Bingkun Bao, Fangting Zuo, Lipeng Yang, Rui Zhang, Huiwen Li, Xinyi Huang, Zhengda Chen, Qingmei Zeng, Renmei Liu, Qiuning Lin, Yuzheng Zhao, Aiming Ren, Linyong Zhu*, Yi Yang*. Large Stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells, Nature Methods, 2023, 20: 1563–1572. 

(2) Zhengda Chen#, Li Jiang#, Xiaoyu Liu, Yajie Shi, Yang Liu, Ling Jiang, Jiaxin Li, Xinyi Huang, Siyu Zhou, Bingkun Bao, Bibi Zhang, Qiuning Lin, Qi Bian, Yuxin Yin, Fangting Zuo, Ni Su, Yun Tang, Yuzheng Zhao, Yi Yang*, Xianjun Chen*, Linyong Zhu*. NirFAP680: A Highly Bright and Stable Large Stokes Shift Near-Infrared Fluorogen-Activating Protein, Nature Methods, 2026, 23: 960–971. 

(3) Fangting Zuo#, Li Jiang#, Ni Su#, Yaqiang Zhang, Bingkun Bao, Limei Wang, Yajie Shi, Huimin Yang, Xinyi Huang, Ruilong Li, Qingmei Zeng, Zhengda Chen, Qiuning Lin, Yingping Zhuang, Yuzheng Zhao, Xianjun Chen*, Linyong Zhu*, Yi Yang*. Imaging the Dynamics of Messenger RNA in Live Cells with Highly Bright and Stable Green Fluorescent RNA, Nature Chemical Biology, 2024, 20: 1272–1281. 

(4) Lu Gao#, Beibei Gao#, Wei Ge, Tianze Sun, Wenshuang Liang, Li Jiang*, Linyong Zhu, Fu Wang*. Deep learning–enhanced single-shot triorganelle STED-FLIM imaging of lipid dynamics in living cells, Science Advances, 2026, 12, eaeh3416. DOI: 10.1126/sciadv.aeh3416. 

(5) Zhengda Chen#, Yajie Shi#, Li Jiang*, Yuxin Yin, Lipeng Yang, Xinyi Huang, Ling Jiang, Wenjie Guo, Xiaoyu Liu, Rong Fu, Huining Liu, Bingkun Bao, Qiuning Lin, Yuzheng Zhao, Xianjun Chen*, Yi Yang*, Linyong Zhu*. A general strategy for developing Si-rhodamine-based fluorogenic dyes for advanced bioimaging and biosensing, Journal of the American Chemical Society, 2026, 148(27): 28764–28775. 

(6) Yajie Shi#, Huiwen Li#, Chenxin Cao, Xin Jiang, Wenjie Guo, Qiuning Lin, Xianjun Chen, Yuzheng Zhao, Li Jiang*, Yi Yang, Linyong Zhu. Structure-Guided Design of Pepper-Binding Fluorophores Through Hydrogen-Bonding Engineering, Angewandte Chemie International Edition, 2026, e4603604. 

(7) Li Jiang#, Fangting Zuo#, Yuanyuan Pan#, Ruilong Li, Yajie Shi, Xinyi Huang, Dasheng Zhang, Yingping Zhuang, Yuzheng Zhao, Qiuning Lin, Yi Yang, Linyong Zhu*, Xianjun Chen*. Bright and Stable Cyan Fluorescent RNA Enables Multicolor RNA Imaging in Live Escherichia coli, Small, 2024, 2405165. 

(8) Xinyi Huang#, Yajie Shi#, Li Jiang*, Wanqi Chen, Bingkun Bao, Tuan Liu, Qinghai Zhou, Jiaxin Li, Qiuning Lin*, Linyong Zhu*. Precise photorelease in living cells by high-viscosity activatable coumarin-based photocages, Chemical Science, 2025, 16: 3611–3619. 

(9) Jiayu Zhang#, Yao Wang#, Xinyi Huang, Haiyu Wu, Qiuning Lin, Hongchen Gu, Li Jiang*, Linyong Zhu, Hong Xu*. Radical-Mediated In Situ Fluorescence Dye Deposition: A Simple Interfacial Signal Amplification Reaction for Ultrasensitive Immunoassay on Barcode Beads, Advanced Science, 2026, e76670.