吴侠 副教授 博士生导师

电子邮箱：wuxia@ecust.edu.cn

通讯地址：上海市徐汇区梅陇路130号华东理工大学科辅助七楼

个人简介：

2008年7月毕业于内蒙古大学，获理学博士学位，留校工作；2009年月赴美国犹他州立大学从事博士后研究；2011-2019年在内蒙古大学省部共建草原家畜生殖调控与繁育国家重点实验室，担任干细胞与重编程课题组组长；2019年调入华东理工大学药学院。曾先后主持国家重大研发计划、国家“863”高科技计划、国家自然等国家和省部级项目。先后获得省部级杰出青年基金、青年创新创业人才、“青年科技英才计划”青年科技骨干等人才类称号。完成成果鉴定1项，申请发明专利30余项，其中PCT专利2项，授权专利7项，参与教材和论著的编著3部，发表研究论文30余篇。担任中国整形美容协会干细胞研究与应用分会理事（上海）、中国细胞生物学会会员。

研究方向与成果：

主要从事基因治疗药物的研发和临床转化研究。以遗传代谢罕见病、关节疾病、老年退行性疾病、癌症等为研究对象，利用腺病毒和腺相关病毒作为递送工具，探讨疾病的作用机制，设计、优化药物载体，进行药理学、毒理学研究，进而推进基因治疗药物的临床转化。2019年与中国医学科学院天津血液病医院合作，开展了亚洲首个血友病B基因治疗药物临床研究。2022年5月，该研究成果发表于国际血液学顶级期刊《The Lancet Haematology》，被评为2022年中国血液学十大研究进展。该研究中的一位受试者在无外部输血情况下，完成全膝关节置换手术，该研究在世界上首次证实基因治疗后能够完全恢复血友病B患者的凝血功能，并发表于《The New England Journal of Medicine》。2023年，《原创基因治疗技术研发与新型血友病诊疗体系的建立和推广》获得天津市科技进步一等奖。此外，开发的四个溶酶体贮积症基因治疗药物已经开展临床转化，并表现出明显的安全性和有效性。

教材与专著：

《动物解剖学、组织学与生殖生物学简明教程》，内蒙古大学出版社，2016.7，ISBN978-7-5665-1016-7

《动物种业科技创新战略研究报告》，科学出版社，2015.6，ISBN978-03-044229-1

《哺乳动物生殖工程学》，科学出版社，2018.10，ISBN978-7-03-058921-7

发表论文（#第一作者；*通讯作者）：

1. Zhang Yanan^#, Tang Dingyue^#, Wang Li, Yang Jing, Wu Xia^*, Xiao Xiao^*, Wang Jianshe^*. Prevention of Portal-Tract Fibrosis in Zfyve19-/- Mouse Model with Adeno-Associated Virus Vector Delivering ZFYVE19. Hum Gene Ther. 2023 Sep 6. doi: 10.1089/hum.2023.041

2. Zhou Kaiyi^#, Yuan Meng, Sun Jiabao, Zhang Feixu, Zong Xiaoying, Li Zhanao, Tang Dingyue, Zhou Lichen, Zheng Jing, Xiao Xiao^*, Wu Xia^*. Sildenafil increases AAV9 transduction after a systemic administration and enhances AAV9-dystrophin therapeutic effect in mdx mice. Gene Ther. 2023 Jul 27. doi: 10.1038/s41434-023-00411-3

3. Xue Feng^#, Li Huiyuan^#, Wu Xia^#, Liu Wei^#, Zhang Feixu, Tang Dingyue, Chen Yunfei, Wang Wentian, Chi Ying, Zheng Jing, Du Zengmin, Jiang Wei, Zhong Chen, Wei Jun, Zhu Ping, Fu Rongfeng, Liu Xiaofan, Chen Lingling, Pei Xiaolei, Sun Junjiang, Cheng Tao^*, Yang Renchi^*, Xiao Xiao^*, Lei Zhang^*. Safety and activity of an engineered, liver-tropic adeno-associated virus vector expressing a hyperactive Padua factor IX administered with prophylactic glucocorticoids in patients with haemophilia B: a single-centre, single-arm, phase 1, pilot trial. Lancet Haematol. 2022, 9(7):e504-e513

4. Zhong Chen^#, Chen Zifeng, Xia Yong, Wu Jun, Zhang Feixu, Cheng Cheng, Wu Xia^*, Zhuang Yingping, Xiao Xiao^*. Treatment of experimental autoimmune encephalomyelitis using AAV gene therapy by blocking T cell costimulatory pathways. Mol Ther Methods Clin Dev. 2022, 27;25:461-475

5. Zhong Chen^#, Jiang Wei, Wang Yefan, Sun Junjiang, Wu Xia^*, Zhuang Yingping, Xiao Xiao^*. Repeated systemic dosing of AAV vectors in immunocompetent mice after blockade of T-cell costimulatory pathways, Human Gene Therapy, 2022, 33(5-6):290-300

6. Wu Jiamei^#, Zhao Kai^#, Du Zengmin, Chen Ying, Zhang Feixu, Jiang Wei, Zheng Jing*, Wu Xia^*, Shen Chengyong^*, Xiao Xiao^*. Systemic effect of FHL1 on neuromuscular junction and myotube formation via insulin-like growth factor and myostatin signaling pathways. Biochem Biophys Res Commun. 2021, 22;537:125-131

7. Wu X^#,*, Hu S, Wang L, Li Yan, Yu H^*.Dynamic changes of histone acetylation and methylation inbovine oocytes, zygotes, and preimplantation embryos.J ExpZool (Mol Dev Evol). 2020;1-12.

8. Tao D^#, Liang J^#, Pan Y, Zhou Y, Feng Y, Zhang L, Xu J, Wang H, He P, Yao J, Zhao Y, Ning Q, Wang W, Jiang W, Zheng J, Wu X^*.  In Vitro and In Vivo Study on the Effect of Lysosome-associated Protein  Transmembrane 4 Beta on the Progression of Breast Cancer. J Breast Cancer. 2019.27;22(3):375-386.

9. Wei Z^#, Yang Z^#, Wu X^*, Yang H^*.  Screening of exosomal miRNAs derived from subcutaneous and visceral  adipose tissues: underlying targets for treatment of obesity and  associated metabolic disorders. Molecular Medicine Reports.  2018;18(3):3314-332

10. Wang Jinze^#, Zhang Feixu^#, Yang Huidi, Wu Huikuan, Cui Rong, Zhao Yunjie, Jiao Cuihua, Wang Xianxin, Liu Xin, Wu Liqiong, Li Guangpeng, Wu Xia^*. Effect of TEAD4 on multilineage differentiation of muscle-derived stem cells. Am J Transl Res. 2018; 10(3): 998-1011.

11. Li S^#, Lu X^#, He H, Cui R, Wang X, Wang X, Wu X^*.A novel culture system robustly maintained pluripotency of embryonic stem cells and accelerated somatic reprogramming by activating Wnt signaling. Am J Transl Res. 2017;9(10):4534-4544.

12. Wu X^#,*, Song M, Yang X, Liu X, Liu K, Jiao C, Wang J, Bai C, Sun G, Liu X, Li G^*. et al. Establishment of bovine embryonic stem cells after knockdown of CDX2. Sci. Rep. 6, 28343; doi: 10.1038/srep28343 (2016).

13. Wu X^#, Ouyang H^#, Duan B, Pang D, Zhang L, Yuan T, Xue L, Ni D, Cheng L, Dong S, Wei Z, Li L, Yu M, Sun Q, Chen D, Lai L, Dai Y, Li G^*. Production of cloned transgenic cow expressing omega-3 fatty acids. Transgenic Res. 2012;21(3):537-43.

14. Wu X^#, Li Y, Xue L, Wang L, Yue Y, Li K, Bou S, Li G, Yu H^*. Multiple histone site epigenetic modifications in nuclear transfer and in vitro fertilized bovine embryos. Zygote. 2011 Feb;19(1):31-45